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gus staining solution  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc gus staining solution
    <t>Histochemical</t> <t>staining</t> of translational fusion constructs (designs in which a promoter drives the expression of a target gene coding sequence translationally fused to <t>GUS,</t> thereby producing a single fusion protein). Scale bars are 5 mm.
    Gus Staining Solution, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gus+staining+solution/L-Xylose/pmc12845159-211-19-46
    Average 95 stars, based on 1 article reviews
    gus staining solution - by Bioz Stars, 2026-11
    95/100 stars

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    1) Product Images from "Identification of a GA-Related Cis -Element Regulating Male Peduncle Elongation in Papaya"

    Article Title: Identification of a GA-Related Cis -Element Regulating Male Peduncle Elongation in Papaya

    Journal: Plants

    doi: 10.3390/plants15020209

    Histochemical staining of translational fusion constructs (designs in which a promoter drives the expression of a target gene coding sequence translationally fused to GUS, thereby producing a single fusion protein). Scale bars are 5 mm.
    Figure Legend Snippet: Histochemical staining of translational fusion constructs (designs in which a promoter drives the expression of a target gene coding sequence translationally fused to GUS, thereby producing a single fusion protein). Scale bars are 5 mm.

    Techniques Used: Staining, Construct, Expressing, Sequencing

    Related Articles

    Staining:

    Article Title: Light regulates xylem cell differentiation via PIF in Arabidopsis
    Article Snippet: .. Seedlings were submerged in the GUS staining solution (50mMphosphate buffer, 0.2% Triton TX-100, 1.5mMpotassium ferrocyanide, 1.5mMpotassium ferricyanide, and 2mM X-Gluc (5-bromo4-chloro-3-indolyl b- D -glucuronide cyclohexylammonium salt dissolved in DMSO; Gold Biotechnology G1281C1) vacuum infiltrated for 5min, and incubated at 37 C in the dark for 2h. ..

    Article Title: A critical role of a eubiotic microbiota in gating proper immunocompetence in Arabidopsis.
    Article Snippet: .. After treatment with SynCom strains for 5 h, seedlings were rinsed with 0.5 ml 50 mM sodium phosphate buffer (pH 7) and submerged in 0.5 ml GUS staining solution (50 mM sodium phosphate (pH 7), 0.5 mM K4[Fe(CN)6], 0.5 mM K3[Fe(CN)6], 1 mM X-Gluc (GoldBio, G1281C) and 0.01% Silwet-L77 (Bioworld, 30630216)). ..

    Article Title: Small molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis.
    Article Snippet: .. Sevenday-old seedlings expressing the FLC::GUS reporter construct were fixed in 90% acetone for 30min at room temperature and stained overnight with GUS staining solution (0.5 mg/mL X-Gluc [Gold Biotechnology; G1281C5], 0.1M sodium phosphate buffer, pH 7.0, 10mM EDTA, 0.5–5.0mM potassium ferricyanide, 0.5–5.0 mM potassium ferrocyanide, Fig. 8 |Global changes inH3K27me3 induced by theDVR06andheat treatments. aMetaplot profiles of the H3K27me3 signal intensity around the genes in the input, V, DVR06-treated V (DVR06), and heat-treated V plants (30°C). ..

    Article Title: Small molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis
    Article Snippet: .. Seven-day-old seedlings expressing the FLC::GUS reporter construct were fixed in 90% acetone for 30 min at room temperature and stained overnight with GUS staining solution (0.5 mg/mL X-Gluc [Gold Biotechnology; G1281C5], 0.1 M sodium phosphate buffer, pH 7.0, 10 mM EDTA, 0.5–5.0 mM potassium ferricyanide, 0.5–5.0 mM potassium ferrocyanide, and 0.1% [v/v] Triton X-100) as described previously The stained plants were cleared overnight in a chloral hydrate solution (chloral hydrate:water:glycerol, 8:2:1 [by volume]) and dehydrated in 60% glycerol overnight. .. Representative photographs were taken under an AXIO Zoom V16 (Carl Zeiss) microscope.

    Article Title: Identification of a GA-Related Cis-Element Regulating Male Peduncle Elongation in Papaya
    Article Snippet: .. Various tissues from T3 transformants (leaf, inflorescence, stem, and pedicels) were collected 44 days after planting, and incubated in GUS staining solution (0.1 M NaPO4 at pH 7.0, 10 mM EDTA, 0.1% Triton X-100, 1 mM K3FE(CN)6 [Thermo Scientific], 2 mM X-Gluc [Gold Biotechnology, St. Louis, MO, USA]) overnight. ..

    Article Title: MAC3A and MAC3B mediate degradation of the transcription factor ERF13 and thus promote lateral root emergence.
    Article Snippet: Lateral roots (LRs) increase root surface area and allow plants greater access to soil water and nutrients.. LR formation is tightly regulated by the phytohormone auxin.. Whereas the transcription factor ETHYLENE-RESPONSIVE ELEMENT BINDING FACTOR13 (ERF13) prevents LR emergence in Arabidopsis (Arabidopsis thaliana), auxin activates MITOGEN-ACTIVATED PROTEIN KINASE14 (MPK14), which leads to ERF13 degradation and ultimately promotes LR emergence.

    Article Title: Small molecules with hydantoin-containing and spiro-like structures promote devernalization in Arabidopsis
    Article Snippet: .. Seven-day-old seedlings expressing the FLC::GUS reporter construct were fixed in 90% acetone for 30 min at room temperature and stained overnight with GUS staining solution (0.5 mg/mL X-Gluc [Gold Biotechnology; G1281C5], 0.1 M sodium phosphate buffer, pH 7.0, 10 mM EDTA, 0.5– 5 mM potassium ferricyanide, 0.5–5 mM potassium ferrocyanide, and 0.1% [v/v] Triton X-100) as described previously45 The stained plants were made transparent overnight in chloral hydrate solution [chloral hydrate: water: glycerol, 8:2:1 (by vol.)] and dehydrated in 60% glycerol overnight. .. Representative photographs were taken under an AXIO Zoom V16 (Zeiss) microscope.

    Article Title: Identification of a GA-Related Cis -Element Regulating Male Peduncle Elongation in Papaya
    Article Snippet: .. Various tissues from T3 transformants (leaf, inflorescence, stem, and pedicels) were collected 44 days after planting, and incubated in GUS staining solution (0.1 M NaPO 4 at pH 7.0, 10 mM EDTA, 0.1% Triton X-100, 1 mM K 3 FE(CN) 6 [Thermo Scientific], 2 mM X-Gluc [Gold Biotechnology, St. Louis, MO, USA]) overnight. ..

    Incubation:

    Article Title: Light regulates xylem cell differentiation via PIF in Arabidopsis
    Article Snippet: .. Seedlings were submerged in the GUS staining solution (50mMphosphate buffer, 0.2% Triton TX-100, 1.5mMpotassium ferrocyanide, 1.5mMpotassium ferricyanide, and 2mM X-Gluc (5-bromo4-chloro-3-indolyl b- D -glucuronide cyclohexylammonium salt dissolved in DMSO; Gold Biotechnology G1281C1) vacuum infiltrated for 5min, and incubated at 37 C in the dark for 2h. ..

    Article Title: Identification of a GA-Related Cis-Element Regulating Male Peduncle Elongation in Papaya
    Article Snippet: .. Various tissues from T3 transformants (leaf, inflorescence, stem, and pedicels) were collected 44 days after planting, and incubated in GUS staining solution (0.1 M NaPO4 at pH 7.0, 10 mM EDTA, 0.1% Triton X-100, 1 mM K3FE(CN)6 [Thermo Scientific], 2 mM X-Gluc [Gold Biotechnology, St. Louis, MO, USA]) overnight. ..

    Article Title: MAC3A and MAC3B mediate degradation of the transcription factor ERF13 and thus promote lateral root emergence.
    Article Snippet: Lateral roots (LRs) increase root surface area and allow plants greater access to soil water and nutrients.. LR formation is tightly regulated by the phytohormone auxin.. Whereas the transcription factor ETHYLENE-RESPONSIVE ELEMENT BINDING FACTOR13 (ERF13) prevents LR emergence in Arabidopsis (Arabidopsis thaliana), auxin activates MITOGEN-ACTIVATED PROTEIN KINASE14 (MPK14), which leads to ERF13 degradation and ultimately promotes LR emergence.

    Article Title: Identification of a GA-Related Cis -Element Regulating Male Peduncle Elongation in Papaya
    Article Snippet: .. Various tissues from T3 transformants (leaf, inflorescence, stem, and pedicels) were collected 44 days after planting, and incubated in GUS staining solution (0.1 M NaPO 4 at pH 7.0, 10 mM EDTA, 0.1% Triton X-100, 1 mM K 3 FE(CN) 6 [Thermo Scientific], 2 mM X-Gluc [Gold Biotechnology, St. Louis, MO, USA]) overnight. ..

    Expressing:

    Article Title: Small molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis.
    Article Snippet: .. Sevenday-old seedlings expressing the FLC::GUS reporter construct were fixed in 90% acetone for 30min at room temperature and stained overnight with GUS staining solution (0.5 mg/mL X-Gluc [Gold Biotechnology; G1281C5], 0.1M sodium phosphate buffer, pH 7.0, 10mM EDTA, 0.5–5.0mM potassium ferricyanide, 0.5–5.0 mM potassium ferrocyanide, Fig. 8 |Global changes inH3K27me3 induced by theDVR06andheat treatments. aMetaplot profiles of the H3K27me3 signal intensity around the genes in the input, V, DVR06-treated V (DVR06), and heat-treated V plants (30°C). ..

    Article Title: Small molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis
    Article Snippet: .. Seven-day-old seedlings expressing the FLC::GUS reporter construct were fixed in 90% acetone for 30 min at room temperature and stained overnight with GUS staining solution (0.5 mg/mL X-Gluc [Gold Biotechnology; G1281C5], 0.1 M sodium phosphate buffer, pH 7.0, 10 mM EDTA, 0.5–5.0 mM potassium ferricyanide, 0.5–5.0 mM potassium ferrocyanide, and 0.1% [v/v] Triton X-100) as described previously The stained plants were cleared overnight in a chloral hydrate solution (chloral hydrate:water:glycerol, 8:2:1 [by volume]) and dehydrated in 60% glycerol overnight. .. Representative photographs were taken under an AXIO Zoom V16 (Carl Zeiss) microscope.

    Article Title: Small molecules with hydantoin-containing and spiro-like structures promote devernalization in Arabidopsis
    Article Snippet: .. Seven-day-old seedlings expressing the FLC::GUS reporter construct were fixed in 90% acetone for 30 min at room temperature and stained overnight with GUS staining solution (0.5 mg/mL X-Gluc [Gold Biotechnology; G1281C5], 0.1 M sodium phosphate buffer, pH 7.0, 10 mM EDTA, 0.5– 5 mM potassium ferricyanide, 0.5–5 mM potassium ferrocyanide, and 0.1% [v/v] Triton X-100) as described previously45 The stained plants were made transparent overnight in chloral hydrate solution [chloral hydrate: water: glycerol, 8:2:1 (by vol.)] and dehydrated in 60% glycerol overnight. .. Representative photographs were taken under an AXIO Zoom V16 (Zeiss) microscope.

    Construct:

    Article Title: Small molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis.
    Article Snippet: .. Sevenday-old seedlings expressing the FLC::GUS reporter construct were fixed in 90% acetone for 30min at room temperature and stained overnight with GUS staining solution (0.5 mg/mL X-Gluc [Gold Biotechnology; G1281C5], 0.1M sodium phosphate buffer, pH 7.0, 10mM EDTA, 0.5–5.0mM potassium ferricyanide, 0.5–5.0 mM potassium ferrocyanide, Fig. 8 |Global changes inH3K27me3 induced by theDVR06andheat treatments. aMetaplot profiles of the H3K27me3 signal intensity around the genes in the input, V, DVR06-treated V (DVR06), and heat-treated V plants (30°C). ..

    Article Title: Small molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis
    Article Snippet: .. Seven-day-old seedlings expressing the FLC::GUS reporter construct were fixed in 90% acetone for 30 min at room temperature and stained overnight with GUS staining solution (0.5 mg/mL X-Gluc [Gold Biotechnology; G1281C5], 0.1 M sodium phosphate buffer, pH 7.0, 10 mM EDTA, 0.5–5.0 mM potassium ferricyanide, 0.5–5.0 mM potassium ferrocyanide, and 0.1% [v/v] Triton X-100) as described previously The stained plants were cleared overnight in a chloral hydrate solution (chloral hydrate:water:glycerol, 8:2:1 [by volume]) and dehydrated in 60% glycerol overnight. .. Representative photographs were taken under an AXIO Zoom V16 (Carl Zeiss) microscope.

    Article Title: Small molecules with hydantoin-containing and spiro-like structures promote devernalization in Arabidopsis
    Article Snippet: .. Seven-day-old seedlings expressing the FLC::GUS reporter construct were fixed in 90% acetone for 30 min at room temperature and stained overnight with GUS staining solution (0.5 mg/mL X-Gluc [Gold Biotechnology; G1281C5], 0.1 M sodium phosphate buffer, pH 7.0, 10 mM EDTA, 0.5– 5 mM potassium ferricyanide, 0.5–5 mM potassium ferrocyanide, and 0.1% [v/v] Triton X-100) as described previously45 The stained plants were made transparent overnight in chloral hydrate solution [chloral hydrate: water: glycerol, 8:2:1 (by vol.)] and dehydrated in 60% glycerol overnight. .. Representative photographs were taken under an AXIO Zoom V16 (Zeiss) microscope.



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    SEM image of young spikes ( a ) and central florets ( b ) from Fielder <t>and</t> <t>AcRR1</t> -OE at the pistil and stamen formation stages. Scale bar, a 1 mm; b 0.5 mm. c Comparison of the florets number that eventually formed in Fielder and AcRR1 -OE. d <t>GUS</t> histochemical assays of Pro AcRR1 : GUS during young spike differentiation. I, floret differentiation stage; II, pistil and stamen formation stage; III, anther connective stage; IV, tetrad stage; V, white anther stage. Scale bar, 100 μm. In ( a ‒ d ) S, spikelet; F, floret primordia; AP, awn primordia; AF, apical floret; CF, central florets; BF, basal florets; A, anther. e , f Cytokinin and IAA contents of Fielder and AcRR1 -OE young spikes at the double ridge stage. g DEGs related to cytokinin degradation and biosynthesis in Fielder and AcRR1 -OE young spikes. h Expression of TaWOX11-2D in young spikes of AcRR1 -OE lines. i Direct binding of AcRR1 to the TaWOX11-2D promoter. j EMSA of the binding of AcRR1-His to the AGATN (A/T/C) motif in the TaWOX11-2D promoter. k ChIP‒qPCR assay of the binding of AcRR1 to the TaWOX11-2D promoter. l Representative spikes and middle spikelets of Jing 411 and the Tawox11-2D mutant. Scale bar, left, 2 cm; right, 1 cm. The grain number per spikelet ( m ) and grain number per spike ( n ) of F 2 TaWOX11-2D DD , TaWOX11-2D Dd and TaWOX11-2D dd plants derived from a cross of the Tawox11-2D mutant with Jing 411. o Direct binding of AcRR1 and wheat orthologs to the TaWOX11-2D promoter. The data are presented as the means ± S.D.s. In ( e ‒ i , k , o ) n = 3 biologically independent replicates. In ( c , m , n ) n represents the number of biologically independent plants. A two-tailed Student’s t test was used to determine P values. Source data are provided as a file.
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    SEM image of young spikes ( a ) and central florets ( b ) from Fielder <t>and</t> <t>AcRR1</t> -OE at the pistil and stamen formation stages. Scale bar, a 1 mm; b 0.5 mm. c Comparison of the florets number that eventually formed in Fielder and AcRR1 -OE. d <t>GUS</t> histochemical assays of Pro AcRR1 : GUS during young spike differentiation. I, floret differentiation stage; II, pistil and stamen formation stage; III, anther connective stage; IV, tetrad stage; V, white anther stage. Scale bar, 100 μm. In ( a ‒ d ) S, spikelet; F, floret primordia; AP, awn primordia; AF, apical floret; CF, central florets; BF, basal florets; A, anther. e , f Cytokinin and IAA contents of Fielder and AcRR1 -OE young spikes at the double ridge stage. g DEGs related to cytokinin degradation and biosynthesis in Fielder and AcRR1 -OE young spikes. h Expression of TaWOX11-2D in young spikes of AcRR1 -OE lines. i Direct binding of AcRR1 to the TaWOX11-2D promoter. j EMSA of the binding of AcRR1-His to the AGATN (A/T/C) motif in the TaWOX11-2D promoter. k ChIP‒qPCR assay of the binding of AcRR1 to the TaWOX11-2D promoter. l Representative spikes and middle spikelets of Jing 411 and the Tawox11-2D mutant. Scale bar, left, 2 cm; right, 1 cm. The grain number per spikelet ( m ) and grain number per spike ( n ) of F 2 TaWOX11-2D DD , TaWOX11-2D Dd and TaWOX11-2D dd plants derived from a cross of the Tawox11-2D mutant with Jing 411. o Direct binding of AcRR1 and wheat orthologs to the TaWOX11-2D promoter. The data are presented as the means ± S.D.s. In ( e ‒ i , k , o ) n = 3 biologically independent replicates. In ( c , m , n ) n represents the number of biologically independent plants. A two-tailed Student’s t test was used to determine P values. Source data are provided as a file.
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    Image Search Results


    Histochemical staining of translational fusion constructs (designs in which a promoter drives the expression of a target gene coding sequence translationally fused to GUS, thereby producing a single fusion protein). Scale bars are 5 mm.

    Journal: Plants

    Article Title: Identification of a GA-Related Cis -Element Regulating Male Peduncle Elongation in Papaya

    doi: 10.3390/plants15020209

    Figure Lengend Snippet: Histochemical staining of translational fusion constructs (designs in which a promoter drives the expression of a target gene coding sequence translationally fused to GUS, thereby producing a single fusion protein). Scale bars are 5 mm.

    Article Snippet: Various tissues from T3 transformants (leaf, inflorescence, stem, and pedicels) were collected 44 days after planting, and incubated in GUS staining solution (0.1 M NaPO 4 at pH 7.0, 10 mM EDTA, 0.1% Triton X-100, 1 mM K 3 FE(CN) 6 [Thermo Scientific], 2 mM X-Gluc [Gold Biotechnology, St. Louis, MO, USA]) overnight.

    Techniques: Staining, Construct, Expressing, Sequencing

    SEM image of young spikes ( a ) and central florets ( b ) from Fielder and AcRR1 -OE at the pistil and stamen formation stages. Scale bar, a 1 mm; b 0.5 mm. c Comparison of the florets number that eventually formed in Fielder and AcRR1 -OE. d GUS histochemical assays of Pro AcRR1 : GUS during young spike differentiation. I, floret differentiation stage; II, pistil and stamen formation stage; III, anther connective stage; IV, tetrad stage; V, white anther stage. Scale bar, 100 μm. In ( a ‒ d ) S, spikelet; F, floret primordia; AP, awn primordia; AF, apical floret; CF, central florets; BF, basal florets; A, anther. e , f Cytokinin and IAA contents of Fielder and AcRR1 -OE young spikes at the double ridge stage. g DEGs related to cytokinin degradation and biosynthesis in Fielder and AcRR1 -OE young spikes. h Expression of TaWOX11-2D in young spikes of AcRR1 -OE lines. i Direct binding of AcRR1 to the TaWOX11-2D promoter. j EMSA of the binding of AcRR1-His to the AGATN (A/T/C) motif in the TaWOX11-2D promoter. k ChIP‒qPCR assay of the binding of AcRR1 to the TaWOX11-2D promoter. l Representative spikes and middle spikelets of Jing 411 and the Tawox11-2D mutant. Scale bar, left, 2 cm; right, 1 cm. The grain number per spikelet ( m ) and grain number per spike ( n ) of F 2 TaWOX11-2D DD , TaWOX11-2D Dd and TaWOX11-2D dd plants derived from a cross of the Tawox11-2D mutant with Jing 411. o Direct binding of AcRR1 and wheat orthologs to the TaWOX11-2D promoter. The data are presented as the means ± S.D.s. In ( e ‒ i , k , o ) n = 3 biologically independent replicates. In ( c , m , n ) n represents the number of biologically independent plants. A two-tailed Student’s t test was used to determine P values. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: AcRR1 of Agropyron cristatum boosts wheat yield by regulating grain number per spike and heading date

    doi: 10.1038/s41467-025-61917-5

    Figure Lengend Snippet: SEM image of young spikes ( a ) and central florets ( b ) from Fielder and AcRR1 -OE at the pistil and stamen formation stages. Scale bar, a 1 mm; b 0.5 mm. c Comparison of the florets number that eventually formed in Fielder and AcRR1 -OE. d GUS histochemical assays of Pro AcRR1 : GUS during young spike differentiation. I, floret differentiation stage; II, pistil and stamen formation stage; III, anther connective stage; IV, tetrad stage; V, white anther stage. Scale bar, 100 μm. In ( a ‒ d ) S, spikelet; F, floret primordia; AP, awn primordia; AF, apical floret; CF, central florets; BF, basal florets; A, anther. e , f Cytokinin and IAA contents of Fielder and AcRR1 -OE young spikes at the double ridge stage. g DEGs related to cytokinin degradation and biosynthesis in Fielder and AcRR1 -OE young spikes. h Expression of TaWOX11-2D in young spikes of AcRR1 -OE lines. i Direct binding of AcRR1 to the TaWOX11-2D promoter. j EMSA of the binding of AcRR1-His to the AGATN (A/T/C) motif in the TaWOX11-2D promoter. k ChIP‒qPCR assay of the binding of AcRR1 to the TaWOX11-2D promoter. l Representative spikes and middle spikelets of Jing 411 and the Tawox11-2D mutant. Scale bar, left, 2 cm; right, 1 cm. The grain number per spikelet ( m ) and grain number per spike ( n ) of F 2 TaWOX11-2D DD , TaWOX11-2D Dd and TaWOX11-2D dd plants derived from a cross of the Tawox11-2D mutant with Jing 411. o Direct binding of AcRR1 and wheat orthologs to the TaWOX11-2D promoter. The data are presented as the means ± S.D.s. In ( e ‒ i , k , o ) n = 3 biologically independent replicates. In ( c , m , n ) n represents the number of biologically independent plants. A two-tailed Student’s t test was used to determine P values. Source data are provided as a file.

    Article Snippet: Histochemical analysis of GUS activity in Pro AcRR1 : GUS transgenic lines was performed with a GUS staining solution (Solarbio, China) according to the manufacturer’s instructions.

    Techniques: Comparison, Expressing, Binding Assay, Mutagenesis, Derivative Assay, Two Tailed Test